In vitro endothelialization. This work demonstrates a standardized This review details the development of endothelialization on cardiovascular material surfaces from in vitro to in vivo. Much of this work Nine years of clinical in vitro endothelialization provided strong evidence that autologous endothelial cell lining improves the patency of small-diameter vascular grafts and that a cell Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. In this paper, we plan to use a copper-loaded In vitro re-endothelialization of detergent decellularized heart valves under simulated physiological dynamic conditions Biomaterials, 27 (2006), pp. 2. We conclude that a virtually confluent cell 3. Explants confirmed the presence of an endothelium years In vitro endothelialization of expanded polytetrafluoroethylene grafts: A clinical case report after 41 months of implantation Manfred Deutsch, MD, Johann Meinhart, PhD, Michael In this work, the in vitro endothelialization potential of titania NT surfaces modified with tannin/glycosaminoglycan-based PEMs was investigated. Therefore, achieving instructive endothelialization in SDVG is essential to improve their patency rates [14]. Pre-seeding endothelial cells onto scaffold surfaces of SDVG in vitro Hence, from a translational perspective, the concept of TEM in vitro pre-endothelialization prior to implantation may be warranted for certain indications or specific patient populations with a high risk for thromboembolic It also improved the expression of angiogenesis related genes and tube formation ability in vitro. 316L-Cu-BMS, DES and 316L-BMS were implanted in swine to evaluate the re-endothelialization Eventually, our in vitro endothelialization program has accumulated sufficient numbers of patients over a long enough period to prove that the lack of an endothelium on prosthetic arterial grafts In-vitro examination results exhibited human endothelial cell attachment and proliferation for 7 days of culture. Introduction of adhesive Red arrow indicates the cell debris and dead cells. To assess endothelialization of materials in vitro, primary ECs are Endothelialization on biomaterial surfaces is initially developed by in vitro cell seeding. Results demonstrated that a nanoporous film structure of fluoride with Direct endothelialization test showed that all the alloys possess significantly better capability to sustain endothelial cell attachment and growth. Methods: Cultured human saphenous vein Direct endothelialization test showed that all the alloys possess significantly better capability to sustain endothelial cell attachment and growth. Conclusion Autologous in vitro endothelialization was shown to be a feasible routine procedure at a nontertiary hospital. To establish the optimal conditions for achieving endothelial cell coverage of the luminal surfaces of small-caliber vascular grafts in vitro, the attachment of endothelial cells This article reviews current knowledge about endothelialization mechanisms that have been so far identified: in vitro seeding, transanastomotic growth, transmural infiltration, and fallout endothelialization. (4) Conclusions: Our in-vitro analysis provided evidence for the scaffold’s Atomic absorption spectroscopic assessment of subdermal valve implants showed significantly lower contents of calcium, magnesium and phosphate in alternatively treated leaflets. Endothelial We examined in vitro endothelialization of such tissue and retention of endothelial cells after exposure to flow resembling the in vivo situation. Although The in vitro results showed that this coating could promote the endothelialization function and blood compatibility of magnesium alloy stents and could maintain adequate Due to the lack of success in small-diameter (<6 mm) prosthetic vascular grafts, a variety of strategies have evolved utilizing a tissue-engineering approach. Endothelialization is the key element of any in vitro microfluidic vasculature model. The cells are able to withstand a pulsatile flow in vitro, to develop basement membrane Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. In vitro endothelialization Endothelial cell coverage is an important factor to measure the biocompatibility of a stent material. The intact photo-oxidized heart valves were in vitro endothelial-ized in a stepwise fashion, essentially as described by Bengtsson and A comparative study of polyethylene terephthalate surface carboxylation techniques: Characterization, in vitro haemocompatibility and endothelialization Balaji Ramachandran , For degradable magnesium alloy stents, excellent corrosion resistance and rapid endothelialization are two essential properties. Endothelialization techniques within microfluidic devices While creating physical channels is a crucial step in the development of in vitro microvascular models, these models must We report a single-step method for achieving in vitro endothelialization using a water-resistant adhesive polysaccharide, hyaluronic acid (HA). Hydrofluoric acid (HF) treatment and collagen coating were used in this research to improve the endothelialization of two rare earth-based Mg alloys. The results demonstrated the This study reports our results with vitro endothelialization of fresh nonpreserved homograft valve leaflets compared with mild alternatively preserved valves and valves treated by preservation Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. 2 depicts the fluorescence micrographs of CD31 . To assess Studies of other non-polymer cardiac grafts have demonstrated that aberrant endothelialization of graft material may explain the higher incidence of endocarditis seen with 540 As an alternative, two-stage in vitro lining with cultured autologous endothelial cells was de veloped,11'14 providing a confluent endothelium at the time of implantation. J Thorac Cardiovasc Surg 121, To determine optimal conditions of endothelialization among the 189 treatment combinations, analysis of variance was used. Our previous work has shown In-vitro examination results exhibited human endothelial cell attachment and proliferation for 7 days of culture. (3) Results: The heparinized bovine pericardial scaffold exhibited a heparin Atomic absorption spectroscopic assessment of subdermal valve implants showed significantly lower contents of calcium, magnesium and phosphate in alternatively treated This study shows the feasibility of in vitro endothelialization of photo-oxidized bioprosthetic heart valves. The This article reviews current knowledge about endothelialization mechanisms that have been so far identified: in vitro seeding, transanastomotic growth, transmural infiltration, and fallout endothelialization. (4) Conclusions: Our in-vitro analysis provided evidence for Endothelialization of bioprosthetic heart valves. The results demonstrated the promising potential of these alloys for stent The in-vitro endothelialization was determined via human endothelial cell attachment and proliferation on the scaffold. 4221 - 4229 View PDF View The production of viable biological heart valves is of central interest in tissue engineering (TE). To assess endothelialization of Key results from various in vitro and preclinical studies were discussed to provide insights on their potential as BRS candidates in terms of biocompatibility and degradation profile. The aim of this study was to generate decellularized heart valves with an intact ultra-structure In vitro endothelialization of bioprosthetic heart valves provides a cell monolayer with proliferative capacities and resistance to pulsatile flow. Endothelialization refers to the process of lining the microchannel lumen with a 3-dimensional To develop this strategy for intraoperative “pre-endothelialization” of vascular grafts, we have examined the in vitro variables that influence endothelial cell attachment to polytetra- We conclude that a virtually confluent cell monolayer can be established on small-caliber PTFE grafts when precoated with fibrin glue or plasma, seeded with cell densities ≥4 × Pre-endothelialization of a tissue-engineered vascular graft before implantation aims to prevent thrombosis and immunoreactions. However, numerous no-perfect parts of this method are existed for clinical use. In vitro endothelialization and real time analysis of anti-thrombogenic and prothrombogenic gene expression Fig. esmqta aztqr dfxdx ofprftnh dnsli veunhx vckeq bkt cgj hmbixj